Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Jun 24;11:3186. doi: 10.1038/s41467-020-17026-6

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 2 — a An experimental scheme for investigating the effect of scan range width on the number of reproducibly detected m/z features: FI-MS analysis of metabolite extracts from serum samples is performed with a series of scan ranges with increasing sizes around a point of 500 m/z (where the mass-stitching revealed numerous reproducible features; in red) and a point of 1650 m/z (where a small number of features are observed; in green). Specifically, using a series of ranges of size 40–700 m/z, in steps of 40 m/z units. b The percent of reproducible m/z features identified around the points of 500 (in red) and 1650 m/z (in green), as a function of the scan range width (out of the total number of features with the minimal 40 m/z scan range). c The distribution of the number of reproducible features found for metabolomics analysis of serum samples by FI-MS in negative mode; and the optimized eight scan ranges represented by vertical lines. d The optimized scan ranges for metabolomics and lipidomics FI-MS-based analysis of serum samples in positive and negative ionization modes. e The number of reproducible m/z features identified with the optimized eight scan ranges (in green), eight uniform scan ranges (in blue), and using a single scan range (in red). *p < 0.004 by two-sample two-sided t-test. Data are mean ± SD, n = 5 independent repetitions of the FI-MS analysis. f The total number of reproducible m/z features (y axis; metabolomics and lipidomics analysis via positive and negative ionization modes) identified when scanning using optimized scan ranges (in green) versus when using uniform width scan ranges (in blue), considering different numbers of scan ranges (x axis); the black horizontal line marks the number of reproducible features observed when scanning using the exhaustive 20 m/z scan ranges. Source data are provided as a Source data file.