Fig. 4. FI-MS-based metabolomics with optimized scan ranges applied for cultured cancer cells.
a The optimized eight scan ranges for metabolomics FI-MS-based analysis of metabolite extracts from cancer cells and from culture media in positive and negative ionization modes. The number of reproducible m/z features observed when utilizing the eight optimized scan ranges (in green), compared with eight uniform scan ranges (in blue), and a single scan range (in red), analyzing metabolite extracts from cancer cell (b) and from culture medium (c). *p < 10−5 by two-sample two-sided t-test. Data are mean values ± SD, n = 5 independent repetitions of the FI-MS analysis. Source data are provided as a Source data file.