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. 2020 Jun 24;7:199. doi: 10.1038/s41597-020-0541-4

Fig. 1.

Fig. 1

Overview of human donor eye dissection, sample collection and downstream analysis. Pairs of whole globe human donor eyes were procured and dissected so that whole corneas were collected (A) prior to making radial cuts down the side of the globe to expose the retina (B). The central retina and adjacent RPE/choroid layers were collected using a 3 mm biopsy punch in the center of each retina while the rod-rich peripheral retina was collected using a 6 mm biopsy punch (C). A rigorous experimental workflow was applied to analyze the mRNA transcriptome of these human ocular samples (D).