Fig. 5. Recombinant IFNβ promotes human CAR T cell apoptosis and inhibitory receptor expression.

CAR and untransduced (UTD) T cells from three healthy donors were cultured in the absence or presence of human IFNβ (0, 0.1, 1, or 10 ng/mL) for 48 h and analyzed for the expression of (a) cleaved caspase 3, (b) TIM3, (c) LAG3, and (d) PD1. e Average expression of individual and combinations of inhibitory receptors for three donors. Data are representative of two independent experiments. Data points indicate the average of two technical replicates for each donor and P-values were determined for all three donors using a two-way repeated measures ANOVA with a Tukey multiple comparisons post-test. Source data are provided in the Source Data File.