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. 2020 Jun 24;11:3196. doi: 10.1038/s41467-020-16838-w

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — Jurkat NFAT reporter assay was used to analyze activation of Prot-FOLR1-TCB (matA or MMP-matA linker) ex vivo by undigested human tumor explants. a Benign tumor of the ovary. b Cancer of the ovary. c Cancer of the ovary. Explants were mechanically cut and then incubated with TCBs and analyzed for CD3 activation using Jurkat NFAT cells. Jurkat NFAT activation is measured in relative luminescence units (RLU). Each symbol indicates the value measured for one biological sample incubated with Jurkat NFAT cells and Prot-FOLR1-TCB MMP-matA site (gray bar), matA site (purple bar), noncleavable site (blue bar) or FOLR1-TCB (red bar). a Each data point shows the mean of technical duplicates measured for one well (n = 2 biological replicates). b Each data point shows the value measured for one well (n = 3 biological replicates). Standard deviation is indicated by error bars. c Each data point shows the mean of technical duplicates measured for one well (n = 2 biological replicates). The dotted lines indicate luminescence for Jurkat NFAT-cells incubated with tumor samples but without any TCB.