Skip to main content
. 2020 Jun 24;11(6):477. doi: 10.1038/s41419-020-2675-x

Fig. 1. Enalapril overcomes 5-FU resistance and potentiates the proliferation-inhibitory effects of 5-FU in CRC cells.

Fig. 1

a HCT116 and SW620 cells were treated with increasing concentrations of enalapril (EP) or 5-FU (FU) for 72 h, and the cell viability was measured by MTT assay. b HCT116 and SW620 cells were treated with EP (100 μM), FU (10 μM), or the two agents combined (E+F) for 72 h, and the cell viability was measured by MTT. c The representative morphology of SW620 cells after 72 h treatment was shown by microscopy at ×200 magnification. d HCT116 and SW620 cells were treated as described in b for 48 h and the cell apoapsis was analyzed by flow cytometry. e The primary CRC cell lines P1, P2, P3, and P4 were treated as described above, and the cell viability was measured by MTT. f The primary CRC cells were treated as described above and, after 48 h, the cell apoapsis was measured by flow cytometry. The data are presented as means ± SD from three separate experiments (n = 8 per group). Statistical analysis performed using two-tailed t-test (**P < 0.01, *P < 0.05).