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. 2020 Jun 24;11(6):477. doi: 10.1038/s41419-020-2675-x

Fig. 3. Enalapril augments the effects of 5-FU against tumor cells migration, invasion, and metastasis in CRC.

Fig. 3

a The migration capacities of SW620 and HCT116 cells were determined using the Transwell chambers after 24 h of treatment with 100 μM EP, 10 μM FU, or E+F (n = 5 per group). Representative photographs of the migrated cells were presented. The number of migrated cells was quantified in ten visual fields at ×200 magnification and the means ± SD are shown in the diagrams. The number of migratory cells in the control is set to 100%. b The invasion capacities of SW620 and HCT116 cells were detected using the Transwell chambers coated with Matrigel after 24 h of treatment as described above (n = 5 per group). The number of migrated cells was quantified in ten visual fields at ×200 magnification. c SW620 cells were injected into the splenic vein of mice and the liver metastasis was assessed after treatment with EP, FU, or E+F (n = 5) for 7 weeks. Representative photograph of the metastatic nodules in the liver was shown and d the number of liver metastatic nodules was counted and plotted. e SW620 cells were orthotopically injected to observe the metastatic spread in vivo after treatment as described above (n = 8 per group). Mice were killed after 8 weeks. f Representative photographs of the metastasis were shown. The data are presented as means ± SD from three separate experiments (a, b). Statistical analysis (a, b, and c) performed using two-tailed t-test and Mann–Whitney test (d, e) (**P < 0.01, *P < 0.05).