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. 2020 Jun 24;11(6):477. doi: 10.1038/s41419-020-2675-x

Fig. 4. Enalapril enhances the effects of 5-FU in suppressing EMT signaling in CRC.

Fig. 4

a Western blot analysis of vimentin, E-cadherin, and Snail in CRC cells treated with EP, FU, or E+F. b Immunohistochemical analysis of Vimentin, E-cadherin, and Snail in tumor tissue samples treated with EP, FU, or E+F (n = 5 per group). c The number of positive cells was quantified in ten visual fields at ×400 magnification and the means ± SD are shown in the diagrams. d SW620 cells were scratched with a pipette tip and then treated with EP, FU, or E+F (n = 6 per group). The images were taken 0 and 24 h after wound formation and the percent of wound closure was analyzed. The data are presented as means ± SD from three separate experiments. Statistical analysis performed using two-tailed t-test (**P < 0.01, *P < 0.05).