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. 2020 Jun 24;10:10286. doi: 10.1038/s41598-020-67175-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Adipocyte Conditioned Medium Promotes Fibroblast Scratch Closure. (A) Representative 10x DIC images at 0 and 24 hours of human dermal fibroblasts in control medium, pre-adipocyte conditioned medium (PCM) and adipocyte conditioned medium (ACM), respectively. Visually identified scratch boundaries are demarcated in red. Scale bar is 500 µm. (B) Quantification of percent scratch closure. Data is presented as mean $\pm$ SEM (n = 6 independent experiments with four samples per group). A two-way ANOVA with Dunnett multiple comparison tests at each time point indicate that fibroblasts exposed to ACM closed the gap significantly faster than fibroblasts in control medium (**p<0.01, *p<0.05). (C) Cell number after 48 hours was not significantly different between groups, indicating that ACM does not enhance proliferation but promotes fibroblasts closure of the scratch.