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. 2020 Jun 24;10:10286. doi: 10.1038/s41598-020-67175-3

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Adipocyte Conditioned Medium Promotes Fibrin Gel Compaction. (A) Representative images of fibroblast-seeded fibrin gels shortly after release and 24 hours later. Gels were cultured in control media, TGF-β1 and AA supplemented media (positive control), pre-adipocyte conditioned media (PCM), or adipocyte conditioned media (ACM). (B) A representative experiment showing percent reduction in initial gel area (i.e., compaction) over time (mean $\pm$ SD, n = 3). (C) Fibrin gel compaction at 24 hours normalized by controls for n = 3 independent experiments, each with 3 sample replicates per group. Data is presented as mean $\pm$ SEM. A one-way ANOVA with Tukey post hoc tests indicates ACM treated gels were significantly reduced in area compared to control (p < 0.001), positive control (p < 0.05), and PCM (p < 0.001).