Fig. 5. Misoriented cell division and centrosome position in Tsc1 mutant cells is not due to intrinsic defects.
a Representative images of 5-day mIMCD3s spheres immunolabelled with γ-tubulin, Par3 and DAPI to show the localization of the centrosomes compared to the apical markers. Scale bar, 10 µm. b Representative images of 5-day mIMCD3s spheres immunolabelled with Arl13b, aPKC, and DAPI to show the localization of the primary cilia compared to the apical markers. Scale bar, 5 µm. c Representative images of cells cultured on L shape patterns and recorded by time-lapse microscopy for the different genotypes. Scale bar, 20 µm. d Distribution of mitotic spindle angles relative to pattern orientation (αb−αa) at anaphase onset. An average of 120 angles from three independent experiments were quantified for each genotype. Horizontal lines represent the mean ± SEM. Source data are provided as a Source Data file.