Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Jun 24;11:3200. doi: 10.1038/s41467-020-16978-z

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 9 — a Representative images of 5-day mIMCD3s spheres of the indicated genotypes immunolabelled with Afadin (red), E-cadherin (green), γ-Tubulin (magenta) antibodies, and DAPI to show the relative localization of Afadin and E-cadherin in interphase and relative to the mitotic spindle poles in 3D spheroids. Insets show higher magnification of one mitotic cell per genotype, to show the relative localization of Afadin and E-cadherin in mitotic cells. In the insets, yellow arrows indicate the localization of the Afadin spots. White arrows indicate localization of the poles of the mitotic spindles. A dotted yellow line indicates Afadin staining in the whole apical domain of mitotic cells. Scale bar, 10 µm. b Percentage of mitosis showing one Afadin spot adjacent to each spindle pole (white), or perturbed afadin localization adjacent to a single spindle pole (dark gray). An average of 10 mitosis were analyzed per experiment per each genotype. The histograms are an average of three independent experiments. Mean ± SEM. c Representative images of 5-day mIMCD3s spheres of the indicated genotypes immunolabelled with α-catenin, ZO-1 and DAPI to show the loss of baso-lateral presence of α-catenin in the Tsc1 KO, with a concomitant appearance of an apical mislocalization relative to the apico-lateral marker ZO-1. Scale bar, 10 µm. d Representative pictures of pre-cystic post-natal day 20 mice, immunolabelled with Afadin antibody to show the localization of the protein in the indicated genotypes. Scale bar, 10 µm. e Representative pictures of pre-cystic post-natal day 20 mice, immunolabelled with α-catenin, ZO-1 and DAPI to show the localization of the proteins in the indicated genotypes. Source data are provided as a Source Data file.