. 2020 Jun 24;10:10288. doi: 10.1038/s41598-020-67215-y

Table 2.

Functional parameters of sodium currents recorded and predicted influence of biophysical properties on channel activity based on observed changes.

		WT-SCN1A	D249E	E788K	E78D	T1934I	M909K
CD (pA/pF)		100 ± 15	102 ± 19	33 ± 6**	67 ± 11	58 ± 7*	20 ± 3***
Voltage dependence of activation	V_1/2 (mV)	−13.7 ± 0.7	−21.3 ± 1.5***	11.1 ± 0.6***	−14.1 ± 1.4	−14.3 ± 1.3	−6.4 ± 1.1***
	V_1/2 ∆ (mV)		−7.6 ± 0.8* ↑**	24.8 ± 0.1* ↓**	−0.4 ± 0.7	−0.6 ± 0.6	7.3 ± 0.4* ↓**
	k (mV)	6.6 ± 0.2	5.1 ± 0.6*	7.4 ± 0.8	6.3 ± 0.6	6.6 ± 0.4	7.0 ± 0.4
	k ∆ (mV)		*1.3 ↑**	0.9	1.0	1.0	0.9
	n	21	9	11	11	12	14
Voltage dependence of steady-state fast inactivation	V_1/2 (mV)	−43.7 ± 3.0	−56.6 ± 2.5*	−58.4 ± 2.2*	−39.0 ± 3.9	−43.2 ± 2.4	−62.4 ± 4.3**
	V_1/2 ∆ (mV)		−12.9 ± *0.5 ↓**	−14.7 ± *0.8 ↓**	4.7 ± 0.9	0.5 ± 0.6	−18.7 ± 1.3 ↓**
	k (mV)	12.3 ± 1.0	9.4 ± 1.4	11.1 ± 0.9	13.4 ± 1.0	13.8 ± 1.6	15.5 ± 2.9
	k ∆ (mV)		1.3	1.1	0.9	0.9	0.8
	Noninactivating part of the current	40 ± 3%	35 ± 5%	42 ± 6%	45 ± 4%	41 ± 4%	42 ± 7%
	n	20	9	5	13	12	6
Voltage dependence of current inactivation evoked by prolonged 1 s conditioning prepulse	V_1/2 (mV)	−44.2 ± 2.5	−52.3 ± 3.9	−50.9 ± 4.2	−39.2 ± 2.9	−43.2 ± 4.0	−53.9 ± 4.1
	V_1/2 ∆ (mV)		−8.1 ± 1.4	−6.7 ± 1.7	5.0 ± 0.4	1.0 ± 1.5	−9.7 ± 1.6
	k (mV)	12.3 ± 0.6	11.4 ± 1.5	16.8 ± 1.8*	13.8 ± 0.9	14.2 ± 1.3	19.8 ± 3.8**
	k ∆ (mV)		1.1	*0.7 ↓**	0.9	0.9	0.6 ↓**
	Noninactivating part of the current	12 ± 0.01%	4 ± 0.01%**	5 ± 0.04%*	11 ± 0.02%	9 ± 0.03%	7 ± 0.01%*
	n	24	8	5	13	12	6
#		***	*******	**	*******	*******	**
Recovery from fast inactivation	τ (ms)¶	9.0 ± 0.4 [59 ± 4%]	12.0 ± *1.5 ↓** [61 ± 7%]	19.8 ± 2.1* ↓** [53 ± 4%]	17.0 ± *3.7↓ [46** ± 5%]*	16.8 ± 2.6 ↓** [48 ± 5%]	24.3 ± 5.5* ↓** [60 ± 7%]
Recovery from fast inactivation	n	23	9	5	13	13	8

Abbreviations: CD, current density; V1/2, voltage of half-maximal activation or inactivation; V1/2 ∆, values represent shift of voltage for half-maximal activation or inactivation relative to the value measured from the wild type channel; k, slope factor of the activation or inactivation curve; k ∆, values indicate multiple of slope factor of the activation or inactivation curve measured from wild type channel. Original values are listed in the column representing the wild type channel. #, represents significant difference between parameter of noninactivating part of the current characterizing steady-state fast inactivation and the same parameter characterizing inactivation evoked by prolonged 1 s conditioning prepulse of the same channel construct; τ represents time constant of monoexponential fit of data from recovery from steady-state fast inactivation; ¶ values in square brackets represent a relative amplitude of inactivated part of the current; ↑, predicted gain of channel activity; ↓, predicted loss of channel activity

Values statistically significantly different from WT-SCN1A are printed in bold and indicated as follows: *p < 0.05; ** p < 0.005; *** p < 0.0001.