Table 4.

The raw, chimera detection, and quality filter reads derived from distillery sludge samples (a) illumina MiSeq sequencing raw reads, (b) pre-processing of sequence read data and (c) summary of singleton OTUs

Sample	Total reads	Passed conserved region filter	Passed mismatch filter
(a)
DS1	1,191,014	1,191,014	1,189,451
DS2	901,757	901,757	900,797

Sample	Consensus read	Chimeric sequences	Pre-processed reads
(b)
DS1	1,189,451	113,453	1,075,931
DS2	900,797	72,237	828,560

(c)
Total reads			1,904,491
Total OTUs picked			621,897
Total singleton OTUs			539,635
Total OTUs after singleton removal			8226

DS1 non-rhizospheric distillery sludge, DS2 rhizospheric distillery sludge