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. 2020 Jun 18;8:510. doi: 10.3389/fbioe.2020.00510

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Copyright © 2020 Monteiro, Sanches-Medeiros, Westmann and Silva-Rocha.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Fold change of complex synthetic promoters at 4 h of growth. Complex promoters' approach could be used as a strategy for the fine-tuning of genetic circuits to perform optimally in a given context. A total of 230 promoter characterizations (74 architectures characterized in different strains and conditions). All promoter details are shown in Table S1. (A) All 230 promoter characterizations (from the lowest to the highest fold change) that were divided into 2 groups of interests. (B) Promoters with fold change between 1 and 10 (group 1: low strength). (C) Promoters with fold change up to 10 (group 2—high strength).