TABLE 1.

Descriptions of the antibodies used for NSPC characterization including antibody specificity, dilution used, and source.

Marker	Specificity	Dilution	Antibody manufacturer
β-iii tubulin	Cytoskeletal protein abundant in neural precursors	1:500	STEMCELL Technologies
GFAP	Intermediate filament cytoskeletal protein expressed in astrocytes and brain stem cells	1:1000	EMD Millipore
O4	Membrane receptor in mature oligodendrocytes	1:500	R&D Systems
BrdU	Cell cycle (S-phase) checkpoint used as a proliferation marker	1:2000	EMD Millipore
Sox2	Transcription factor necessary for the self-renewal of neural stem cells	1:1000	Abcam

GFAP, glial fibrillary acidic protein; BrdU, bromodeoxyuridine; Sox2, SRY-related HMG box.