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. 2020 Jun 18;11:1295. doi: 10.3389/fmicb.2020.01295

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Copyright © 2020 McClure, Conly, Obasuyi, Ward, Ugarte-Torres, Louie and Zhang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Agarose gel electrophoresis of round 1 and round 2 PCR reactions. (A) No PCR products corresponding to the correct sized product were seen following 10 cycles of long range amplification. Lanes from left to right correspond to the following samples: 1 Kb + marker (Invitrogen), High molecular weight marker (Invitrogen), SCCmec I–XI, no template negative control. (B) Round 2 orfX and mecA reactions show the correct sized amplicon in the orfX reaction, while most types produce weak and multiple bands in the mecA reaction. Lanes from left to right in each gel correspond to the following samples: 1 Kb + marker (Invitrogen), SCCmec I-XI, no template negative control.