Figure 2.

Flow cytometry gating strategy; singlets were identified using FSC-A and FSC-H, and granulocytes were gated based on size and granularity. The markers CD11b, Ly6C, and F4/80 were used to identify subpopulations of macrophages and Ly6G was used to identify neutrophils (A). C57BL/6 mice, vaccinated subcutaneously with 5 × 10⁴ CFU BCG Pasteur, showed an increase of CD11b⁺ cells in the lungs and a lower percentage of those cells are positive for the marker F4/80 and Ly6C at day seven post-vaccination (B). The lungs from BCG vaccinated mice also showed an increase in the percentage of CD11b⁺ cells that were also F4/80⁺ (C). BCG vaccinated mice also showed an increase in the number of double positive cells in the lungs for CD11b and CD14 expression (D). Lungs from vaccinated mice also had a small increase in CD11b⁺ Ly6G⁺ neutrophils (E). N = 5 mice per group and are representative of multiple iterations, *p ≤ 0.05 **p ≤ 0.01 (Wilcoxon rank sum test).