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Expression of DprA and truncated DprA proteins in D. radiodurans. Strains GY 16054 (dprA::HA-kan), GY 16201 (ΔNterdprA::HA-kan), and GY 16058 (dprAΔCter::HA-kan) cell extracts were subjected to SDS-PAGE and analyzed by western blotting with anti-HA antibodies. DprA::HA (38 kDa) and DprAΔCter::HA proteins (35 kDa) are indicated with arrows. The area where ΔNterDprA::HA protein (33 kDa) is expected is indicated by a dotted line.
