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. 2020 Jun 18;11:785. doi: 10.3389/fpls.2020.00785

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Copyright © 2020 Xu, Tang, Wang, Ge, Sun, Qi, He, Zhou, Chen, Xu, Ma and Chen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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SiMYB56 activates the expression of lignin synthesis related genes under drought conditions. (A) Expression analysis of lignin biosynthesis related genes in wild-type controls and transgenic rice plants. Entire 2-week-old SiMYB56-overexpressing and wild-type seedlings, growing under normal condition and 10% PEG6000 treatment, were harvested for RNA isolation. Transcript level was quantified by qRT-PCR. Values shown are means ± SD (n = 3). (B) Transcriptional activity assays in Nicotiana benthamiana leaves. SiMYB56 can activate the transcription of 4CL5 and F5H1. Four-week-old Nicotiana benthamiana plants were prepared for Agrobacterium-mediated transient expression, and generally the third through fifth leaf of each plant were analyzed. In each analysis, five independent Nicotiana benthamiana leaves were infiltrated and analyzed, and totally three biological replications were performed with quantification.