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. 2020 Jun 18;11:785. doi: 10.3389/fpls.2020.00785

FIGURE 8.

FIGURE 8

Overexpression of SiMYB56 significantly improves ABA accumulation in transgenic rice seeds and activates the expression of ABA signaling pathway related genes under drought condition. (A) Seeds of wild-type controls and transgenic rice plants were germinated on plates containing different ABA concentrations. Germination rates of SiMYB56-overexpressing and wide-type seeds under 0 μM (B), 2.5 μM (C), and 5 μM (D) ABA treatments (n = 3, 16 seeds in each replicate). (E) ABA content of sprouts from germinated seeds of transgenic rice plants and wide-type controls under 0 μM ABA treatments. Values shown are means ± SD (n = 3). (F) Expression analysis of ABA signaling pathway related genes in wild-type controls and transgenic rice plants. Entire 2-week-old SiMYB56-overexpressing and wild-type seedlings, grown under normal condition and 10% PEG6000 treatments, were harvested for RNA isolation. Transcript level was quantified by qRT-PCR. Values shown are means ± SD (n = 3).