FIGURE 3.

Lack of inflammatory morphology of microglia in 12-week-old R6/1 mice. (A) Illustrative Iba-1 immunostaining images of the striatum from WT and pre-symptomatic R6/1 mice (12 weeks of age). Scale bar: 40 μm. (B) Quantitative analysis of the number of Iba-1 positive cells per section. Data are expressed as a percentage of WT values (WT n = 3 female + 5 male mice, R6/1 n = 3 female + 3 male mice). (C) Illustrative images of 3DMorph analysis of individual microglial cells isolated from confocal microphotographs. From each full cell volume, 3D skeletons were generated and then processed to obtain an image of either major branches or branch end points. In skeleton figures, colors indicate order of connectivity (red = primary, yellow = secondary, green = tertiary, and blue = connected to end point). (D) Quantitative analysis of the microglial cell volume, territory occupied by each cell, microglial cell complexity (cell territory/cell volume), number and longitude of cell processes, and number of endpoints. (WT n = 3 female + 4 male, R6/1 n = 3 female + 3 male mice).