Skip to main content
. 2020 Jun 18;14:163. doi: 10.3389/fncel.2020.00163

FIGURE 4.

FIGURE 4

Microglia increases PSD-95-positive puncta engulfment in 12-week-old R6/1 mice. (A) Illustrative confocal images of PSD-95 immunostaining and quantitative analysis of the number of PSD-95-positive puncta per section in the striatum of WT and R6/1 mice of 12 and 20 weeks of age. Graph data are expressed as a percentage of WT values. (B) Illustrative confocal images of Iba-1 (green) and PSD-95 (red) double immunostaining in the striatum of WT and R6/1 mice at 12 weeks of age. (C) Three-dimensional microglia reconstruction and surface rendering by means of Imaris software showing larger relative numbers of PSD-95 positive puncta inside Iba-1 positive cells in R6/1 mice. Data are expressed as a percentage of engulfed puncta in WT striatum. *p = 0.0158; **p = 0.003, two-tailed unpaired t-test (n = 2 female + 3 male mice/group). Scale bar: 15 μm in a and b; 7 μm in c.