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. 2020 Jun 18;8:445. doi: 10.3389/fcell.2020.00445

FIGURE 5.

FIGURE 5

IGF1/Sox9 and IGF1/Osterix immunofluorescence double staining in the TMJ in different animal models. (A–D) DAPI staining. (E,F,H) Sox9 staining. (G) Osterix staining. (I–L) IGF1 staining. (M–P) Merged image of DAPI, Sox9/Osterix, and IGF1 staining. ① The cell counts of different markers in the normal TMJ, with the count of DAPI accepted as 100 and the counts of other markers calculated and compared with the count of DAPI. ② The relative counts of IGF1-positive cells and costained cells were higher in the injured condylar cartilage in the first animal model than those in the normal TMJ (∗∗p < 0.01). ③ The relative counts of IGF1-positive cells and costained cells were lower in the injured subchondral bone in the second animal model than those in the first animal model (∗∗p < 0.01). ④ The relative counts of IGF1-positive cells and costained cells were also lower in the injured condylar cartilage in the third animal model than those in the first animal model (∗∗p < 0.01). AD, articular disc; CO, condyle. Scale bar: 50 μm.