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. 2020 Jun 18;8:445. doi: 10.3389/fcell.2020.00445

FIGURE 8.

FIGURE 8

TRPV4/Sox9 immunofluorescence double staining in the TMJ in different animal models. (A–C) H&E examination revealed the structure of TMJ in different animal models. (D–F) DAPI staining. (G–I) TRPV4 staining. (J–L) Sox9 staining. (M–O) Merge of DAPI, Sox9, and TRPV4 staining. ① The cell counts of different markers in the normal TMJ, with the count of DAPI accepted as 100 and the counts of other markers calculated and compared with the count of DAPI. ② The relative counts of TRPV4-positive cells and TRPV4/Sox9 costained cells were higher in the injured condylar cartilage in the first animal model than in the normal TMJ (∗∗p < 0.01). ③ The relative counts of TRPV4-positive cells and TRPV4/Sox9 costained cells were lower in the injured condyle cartilage in the third animal model than in the first animal model (∗∗p < 0.01). AD, articular disc; CO, condyle. Scale bar: 50 μm.