TABLE 10.
BioFire PN panel AMR discrepancy investigation
| Analyte | Specimena | No. of samples with result |
|||
|---|---|---|---|---|---|
| False positive |
False negative |
||||
| Total | rTPb | Total | cFNc | ||
| AMR markers | |||||
| mecA/mecC + MREJ | BAL | 6 | 5d | 5 | 5 |
| SPU | 13 | 13 | 4 | 4 | |
| KPC | BAL | 1 | 1 | ||
| SPU | |||||
| NDM | BAL | 1 | 0 | 1 | 1 |
| SPU | |||||
| VIM | BAL | ||||
| SPU | 1 | 0 | |||
| CTX-M | BAL | 1 | 1 | ||
| SPU | 1 | 0 | 2 | 1 | |
| Viruses | |||||
| Adenovirus | BAL | ||||
| SPU | 2 | 1 | 4 | 4 | |
| Coronavirus | BAL | 13 | 8 | 3 | 2 |
| SPU | 6 | 3 | 4 | 4 | |
| Human metapneumovirus | BAL | 1 | 0 | ||
| SPU | 1 | 0 | 1 | 1 | |
| Rhinovirus/enterovirus | BAL | 11 | 8 | 2 | 2 |
| SPU | 13 | 12 | |||
| Influenza A virus | BAL | 3 | 3 | ||
| SPU | 3 | 2 | |||
| Influenza B virus | BAL | 1 | 1 | 1 | 1 |
| SPU | 2 | 0 | |||
| Parainfluenza virus | BAL | 2 | 2 | 2 | 2 |
| SPU | 2 | 1 | 1 | 1 | |
| RSV | BAL | ||||
| SPU | 4 | 4 | |||
| Atypical bacteria | |||||
| C. pneumoniae | BAL | 1 | 0 | ||
| SPU | |||||
| L. pneumophila | BAL | ||||
| SPU | 1 | 1 | |||
| M. pneumoniae | BAL | 1 | 0 | ||
| SPU | 1 | 0 | |||
| Total | 89 | 64 | 33 | 30 | |
a
SPU, sputum.
b
rTP, resolved true positive; evidence of AMR presence by independent molecular method confirming BioFire PN panel correct result.
c
cFN, confirmed false negative; evidence of AMR presence by independent molecular method confirming BioFire PN panel incorrect result.
d
Insufficient leftover volume for discrepancy investigation of one specimen.