TABLE 11.

Performance of the BioFire PN panel for atypical bacteria and viruses compared to independent PCR and sequencing

Analyte	Sourcea	Positive percent agreement		Negative percent agreement
		TP/(TP+FN)	% (95% CI)	TN/(TN+FP)	95% CI
Atypical bacteria
C. pneumoniae	BAL	0/0		844/845	99.9 (99.3–100)
	SPU	0/0		835/835	100 (99.5–100)
L. pneumophila	BAL	2/2	100 (34.2–100)	833/833	100 (99.5–100)
	SPU	0/1		826/826	100 (99.5–100)
M. pneumoniae	BAL	3/3	100 (43.9–100)	841/842	99.9 (99.3–100)
	SPU	7/8	87.5 (52.9–97.8)	827/827	100 (99.5–100)
Viruses
Adenovirus	BAL	8/8	100 (67.6–100)	837/837	100 (99.5–100)
	SPU	13/17	76.5 (52.7–90.4)	815/817	99.8 (99.1–99.9)
Coronavirus	BAL	18/21	85.7 (65.4–95.0)	810/823	98.4 (97.3–99.1)
	SPU	28/32	87.5 (71.9–95.0)	796/802	99.3 (98.4–99.7)
Human metapneumovirus	BAL	8/8	100 (67.6–100)	836/837	99.9 (99.3–100)
	SPU	20/21	95.2 (77.3–99.2)	812/813	99.9 (99.3–100)
Rhinovirus/enterovirus	BAL	52/54	96.3 (87.5–99.0)	771/782	98.6 (97.5–99.2)
	SPU	96/96	100 (96.2–100)	717/730	98.2 (97.0–99.0)
Influenza A virus	BAL	10/10	100 (72.2–100)	830/833	99.6 (98.9–99.9)
	SPU	13/13	100 (77.2–100)	819/822	99.6 (98.9–99.9)
Influenza B virus	BAL	5/6	83.3 (43.6–97)	837/838	99.9 (99.3–100)
	SPU	12/12	100 (75.8–100)	821/823	99.8 (99.1–99.9)
MERS-CoV	BAL	0/0		846/846	100 (99.5–100)
	SPU	0/0		836/836	100 (99.5–100)
Parainfluenza virus	BAL	16/18	88.9 (67.2–96.9)	824/826	99.8 (99.1–99.9)
	SPU	28/29	96.6 (82.8–99.4)	804/806	99.8 (99.1–99.9)
RSV	BAL	3/3	100 (43.9–100)	841/841	100 (99.5–100)
	SPU	43/43	100 (91.8–100)	787/791	99.5 (98.7–99.8)

^aSPU, sputum.