TABLE 2.
Summary of discrepant NG-Test Carba 5 resultsa
| Site | Organism identification | Disk diffusion results |
mCIM |
eCIM |
NG-Test Carba 5 result on: |
Xpert Carba-R result | Alternative PCR + sequencing result | Final interpretation post-discrepant analysis | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| ETP |
IPM |
MEM |
||||||||||||||
| ZD (mm) | Interpretation | ZD (mm) | Interpretation | ZD (mm) | Interpretation | ZD (mm) | Result | ZD (mm) | Result | Blood agar | MacConkey agar | |||||
| 1 | Enterobacter cloacae | 16 | R | 13 | R | 18 | R | 6 | Pos | 6 | Neg | IMP | IMP | Neg | IMP-8 | True positive |
| 2 | Enterobacter cloacae | 17 | R | 19 | R | 19 | R | 6 | Pos | 6 | Neg | IMP | IMP | Neg | IMP-8 | True positive |
| 3 | Serratia marcescens | 6 | R | 6 | R | 9 | R | 6 | Pos | 19 | Pos | IMP | IMP | Neg | IMP-8 | True positive |
| 2 | Klebsiella oxytoca | 10 | R | 6 | R | 10 | R | 6 | Pos | 6 | Neg | KPC, NDM | KPC, NDM | KPC | KPC, NDM | True positive |
| 1 | Pseudomonas aeruginosa | NA | NA | 16 | R | 6 | R | 6 | Pos | NA | NA | IMP | IMP | Neg | IMP-7 | True positive |
| 3 | Pseudomonas aeruginosa | NA | NA | 6 | R | 6 | R | 6 | Pos | NA | NA | IMP | IMP | Neg | IMP-15 | True positive |
| 2 | Pseudomonas aeruginosa | NA | NA | 11 | R | 6 | R | 6 | Pos | NA | NA | IMP | IMP | Neg | IMP-19 | True positive |
a
ZD, disk diffusion zone diameter; R, resistant; ETP, ertapenem; IPM, imipenem; MEM, meropenem; eCIM, EDTA-modified carbapenem inactivation method; mCIM, modified carbapenem inactivation method; Neg, negative; Pos, positive; NA, not applicable.