TABLE 6.
Qualitative comparison of viral targets detected by BioFire PN panel and standard-of-care testing in 259 BAL specimensa
| Target(s) detected by BioFire PN panel | No. of BAL specimens | No. (%) of BAL specimens with SOC test order for viral target(s) detected | Agreement (%) between SOC and PN panel for viral target(s) detected | No. (%) of BAL specimens with bacterial target codetection by PN panel |
|---|---|---|---|---|
| hRV/EV | 17 | 6 (35) | 6/6 (100) | 7 (41) |
| CoV | 9 | 2 (22) | 2/2 (100) | 2 (22) |
| FluA | 5 | 0 (0) | NA | 2 (40) |
| FluB | 2 | 1 (50) | 1/1 (100) | 1 (50) |
| RSV | 2 | 0 (0) | NA | 0 (0) |
| PIV | 3 | 1 (33) | 1/1 (100) | 1 (33) |
| hMPV | 1 | 0 (0) | NA | 1 (100) |
| AdV | 1 | 0 (0) | NA | 0 (0) |
| CoV+hMPV | 1 | 1 (100) | 1/1 (100) | 1 (100) |
| hRV/EV+PIV | 3 | 0 (0) | NA | 2 (66) |
| hRV/EV+CoV | 1 | 0 (0) | NA | 1 (100) |
| hMPV+FluA+CoV | 1 | 0 (0) | NA | 0 (0) |
| None detected | 213 | 79 (37) | 76/79b (96) | 80 (38) |
| Total | 259 | 90 (34.7) | 87/90 (96.7) | 98 (37.8) |
BAL, bronchoalveolar lavage; SOC, standard-of-care molecular test; hRV/EV, human rhinovirus/enterovirus; CoV, coronavirus; FluA, influenza A virus; FluB, influenza B virus; RSV, respiratory syncytial virus; PIV, parainfluenza virus; hMPV, human metapneumovirus; AdV, adenovirus.
Three BAL specimens were reported as positive for an on-panel target by the standard-of-care test that was not detected by the BioFire PN panel. This included one specimen reported as positive for PIV-3 (BioFire FilmArray Respiratory panel), one specimen positive for FluA (Cepheid Xpert Flu/RSV assay), and one specimen positive for AdV (GenMark XT-8 system).