FIG 2.
Capsule cross-regulation modulates biofilm formation in B. thetaiotaomicron. (A) The 96-well plate crystal violet biofilm assay after 48 h of growth in BHIS. (B) Organization of B. thetaiotaomicron capsular operon 4 (CPS4) with identified transposon insertion points in the first two genes of the operon (BT1358 and BT1357), coding regulators of capsular biosynthesis. (C) The 96-well plate crystal violet biofilm assay after 48 h of growth in BHIS. The Mean of the WT is adjusted to 100%. (D) The 96-well plate crystal violet biofilm assay after 48 h of growth in BHIS. In panels A, C, and D, the mean of the WT is adjusted to 100%. In the min-max boxplot of 6 to 9 biological replicates for each strain, each replicate is the mean of two technical replicates. **, P < 0.005; Mann-Whitney test. upxY, upxYBT1358; upxZ, upxZBT1357.