FIG 3.
Capsule expression in B. thetaiotaomicron is heterogenous and has consequences for biofilm formation. (A and D) The 96-well plate biofilm assay after 48 h of growth in BHIS. The mean of the WT is adjusted to 100%. In the min-max boxplot of 6 biological replicates for each strain, each replicate is the mean of two technical replicates. **, P < 0.005; Mann-Whitney test, comparing the indicated mutant to the WT. The pictures shown under the boxplot in panel A correspond to representative CV-stained microtiter wells after resuspension of the biofilm. (B) Transmission electron microscopy (TEM) images of overnight cultures fixed with ferritin. Arrows indicate some examples of acapsulated cells. (C) Percentage of acapsulated cells of indicated strains counted on TEM pictures. For each strain, at least 100 cells were counted. ***, P < 0.0005; prop.test (R). upxY, upxYBT1358; upxZ, upxZBT1357.