FIG 3.

Effect of exposure to depleted zymosan during in vivo differentiation of Lin⁻ progenitor cells on macrophage cytokine response. (A) Schematic protocol of cell transplantation and stimulation (see Materials and Methods). (B) Macrophages were stimulated with 100 ng/ml of Pam₃CSK₄, and TNF-α and IL-6 levels in 24-h culture supernatants were assessed by ELISA. Triplicate samples were analyzed in each assay. Results are expressed as means ± SDs of pooled data from two experiments. ***, P < 0.001 with respect to cytokine production by macrophages derived from Lin⁻ cells differentiated with M-CSF only, in the absence of depleted zymosan.