TABLE 1.
Bacterial strains used in this study
| Strain | Relevant genotype | Source or reference |
|---|---|---|
| RAM1292 | MC4100 (Δ[argF-lac]169 λ– e14– flhD5301 Δ[fruK-yeiR]725 relA1 rpsL150 (Strr) rbsR22 Δ[fimB-fimE]632 deoC1) Δara714 | 61 |
| RAM1541 | RAM1292 envZR397L | 29 |
| RAM2697 | RAM1292 Δfur::scar | This study |
| RAM2698 | RAM1292 envZR397L Δfur::scar | This study |
| RAM2699 | RAM1292 ΔompC::scar | This study |
| RAM2700 | RAM1292 envZR397L ΔompC::scar | This study |
| RAM2701 | RAM1292 ΔfeoB::scar | This study |
| RAM2702 | RAM1292 envZR397L ΔfeoB::scar | This study |
| RAM2703 | RAM1292 envZR397L ΔfeoB::scar ΔompC::scar | This study |
| RAM2704 | RAM1292 ΔompR::scar | This study |
| RAM2705 | RAM1292 ΔompR::scar pBAD24-ompR6His | This study |
| RAM2707 | RAM1292 ΔompR::scar pBAD24 | This study |
| RAM2708 | RAM1292 pBAD24 | This study |
| RAM2709 | RAM1292 pBAD24-ompR6His | This study |
| RAM2711 | RAM1292 ΔfeoA::lacZ-Kmr | This study |
| RAM2712 | RAM1292 ΔfeoA::lacZ-Kmr envZR397L | This study |
| RAM2713 | RAM1292 ΔfeoA::lacZ-Kmr Δfur::scar | This study |
| RAM2714 | RAM1292 ΔfeoA::lacZ-Kmr Δfur::scar envZR397L | This study |
| RAM2715 | BL21(DE3) pET24d-ompR6His | This study |
| RAM2469 | RAM1292 ΔfepA::lacZ-Kmr | This study |
| RAM2470 | RAM1292 ΔfepA::lacZ-Kmr envZR397L | This study |
| RAM2471 | RAM1292 ΔfepA::lacZ-Kmr Δfur | This study |
| RAM2472 | RAM1292 ΔfepA::lacZ-Kmr Δfur envZR397L | This study |
| RAM2473 | RAM2469 ΔompC::Cmr | This study |
| RAM2474 | RAM2469 ΔfeoB::Cmr | This study |
| RAM2475 | RAM2470 ΔompC::Cmr | This study |
| RAM2476 | RAM2470 ΔfeoB::Cmr | This study |
| RAM2477 | RAM2469 ΔompF::Cmr | This study |
| RAM2478 | RAM2470 ΔompF::Cmr | This study |
| RAM2505 | RAM1292 ΔtonB::Kmr | This study |
| RAM2553 | RAM1292 ΔaroB::Kmr | This study |
| RAM2572 | RAM2505 ΔtonB::scar (via pCP20) | This study |
| RAM2574 | RAM2572 ΔaroB::Kmr | This study |
| RAM2623 | RAM2553 ΔaroB::scar | This study |
| RAM2625 | RAM2574 ΔaroB::scar (via pCP20) | This study |
| RAM2765 | RAM2572 malPQ::Tn10-ompR+ | This study |
| RAM2766 | RAM2572 malPQ::Tn10-ompR101 | This study |
| RAM2767 | RAM2572 ΔompR::Kmr | This study |
| RAM2768 | RAM2572 ΔompF::Kmr | This study |
| RAM2769 | RAM2572 ΔompC::Cmr | This study |
| RAM2771 | RAM2574 malPQ::Tn10-ompR+ | This study |
| RAM2772 | RAM2574 malPQ::Tn10-ompR101 | This study |
| RAM2790 | RAM2768 (pTrc99A-ompF) | This study |
| RAM2792 | RAM2769 (pTrc99A-ompC) | This study |
| RAM2795 | RAM2790 ΔompC::Cmr | This study |
| RAM2796 | RAM2792 ΔompF::Kmr | This study |
| RAM2920 | RAM2469 Cmr linked to envZ+ | This study |
| RAM2921 | RAM2470 Cmr linked to envZR397L | This study |
| RAM2922 | RAM2799 Cmr linked to ompRD55Y envZR397L | This study |
| RAM2924 | RAM2711 Cmr linked to envZ+ | This study |
| RAM2925 | RAM2711 Cmr linked to envZR397L | This study |
| RAM2926 | RAM2711 Cmr linked to ompRD55Y envZR397L | This study |
| RAM2928 | RAM2711 pBAD24 (Apr) | This study |
| RAM2932 | RAM2711 pBAD24-ompR6His | This study |