FIG 2.

Production of pectate lyase and expression of evf are dependent on both quorum sensing and the GAC system. (A) Pectate lyase activity in cell-free supernatants of WT Ecc15 and expI and gacA mutants at 6 h of growth in LB plus 0.4% PGA, n = 10. (B) Potato maceration quantification (grams) in potatoes infected with WT Ecc15 and expI, gacA, and evf mutants, 48 h postinfection, n = 8. (C) Pevf::gfp expression in WT Ecc15, expI and gacA mutants, and expI+AHLs at 6 h of growth in LB + Spec, n = 5. Complementation with AHLs (expI+AHLs) was performed with a mixture of 1 μM 3-oxo-C6-HSL and 3-oxo-C8-HSL. Growth curves of the strains used are shown in Fig. S1. Error bars represent standard deviations of the means. For each panel, results from a representative experiment from three independent experiments are shown (the results from the other two experiments are shown in Fig. S2). Results of statistical analysis taking the data of all three experiments are shown in Fig. S2. a.u., arbitrary units.