FIG 4.

Ecc15 loads are higher in D. melanogaster larvae orally infected with the WT than in those infected with mutants impaired in evf expression. D. melanogaster L3 stage larvae were infected with WT Ecc15 and evf, expI, and gacA mutants for 30 min and then transferred to fresh media. Following the infection period, CFU levels of Ecc15 were measured at the specified time points. Each dot represents CFU of one single larvae (5 larvae per time point). The time point indicated as 0 h after infection corresponds to 30 min of confined exposure to 200 μl of an OD₆₀₀ of 200. Results from a representative experiment from three independent experiments are shown (results from the other two experiments are shown in Fig. S5). Results of statistical analysis of the comparisons of data from the entire infection period for each condition tested in all three experiments are shown in Fig. S6.