FIG 6.

Coactivation of evf and pelA expression in plant infections. Potatoes were infected for 6, 24, or 48 h with a WT Ecc15 strain or an expI mutant carrying an evf or pelA gfp reporter plasmid with a constitutive mCherry promoter. At each specified time point, pelA or evf expression, CFU levels, and weight of macerated plant tissue were determined. (A and B) P_evf::gfp expression (A) and P_pelA::gfp expression (B) in potatoes infected with WT Ecc15 or expI mutant. (C) CFU of WT Ecc15 or expI mutant expressing mCherry and carrying the P_evf::gfp (green boxes) or the P_pelA::gfp (red boxes) reporter plasmids. (D) Potato maceration (in grams) in potatoes infected with WT Ecc15 or expI mutant. Each dot represents an independent potato, n = 5. Results are from a representative experiment of two independent experiments (results from the second experiment are shown in Fig. S8). Results of statistical analysis of comparisons of data from the entire infection period for each condition tested using the data from both experiments are shown in Fig. S8.