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. 2020 Jun 23;11(3):e01293-20. doi: 10.1128/mBio.01293-20

FIG 2.

FIG 2

Stimulation of pathogen-associated molecular patterns upregulates CD47 surface expression. (A) MFI of CD47 surface expression on human PBMC monocytes and dendritic cells after 48 h of stimulation with either 1 μg/ml MDP or 1 μg/ml CL264 or with no stimulation. The results are from one of three experiments with two different donors. All 3 experiments showed consistent effects. Statistics were done by a paired two-way t test with Bonferroni correction. (B) CD47 MFI on human total PBMCs from 4 separate donors stimulated with titrated concentrations of R848 from 0.1 μg/ml to 10 μg/ml, as indicated, for 48 h. Statistics were done by a paired two-way t test with Bonferroni correction. (C) Mice (n = 5/group) were injected intraperitoneally with 1 mg/kg R848 for 3 days, and on day 3, splenocytes were isolated and macrophages and DCs were analyzed for CD47 MFI. Statistics were done by an unpaired two-way t test. (ns, P > 0.05; *, P < 0.05; **, P < 0.01; ***, P < 0.001). Error bars represent SEM.