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. Author manuscript; available in PMC: 2021 Apr 1.
Published in final edited form as: Neurobiol Dis. 2020 Jan 21;137:104758. doi: 10.1016/j.nbd.2020.104758

Figure 2: Mouse pup and adult behavioral phenotyping of Iqsec2−/Y animals.

Figure 2:

A. Panels from left to right show comparison of Iqsec2 pups at PND8 and graphs for developmental milestones. Analysis of developmental milestone markers revealed genotype differences on measures of body weight but not righting reflex Number of ultrasonic vocalizations emitted by pups separated from the nest did not differ significantly among genotypes but were fewer in Iqsec2−/Y mice (n=10) compared to Iqsec2+/Y mice (n=16); Two-way ANOVA, Sidak’s multiple comparison test, **p<0.01, ***p< 0.001; error bars indicate ± SEM. Adult IQSEC2 animals are hyperambulatory (B), hyperanxious (C) and display defects in social interaction (D). B. Representative tracks of Iqsec2+/Y and Iqsec2−/Y mice during a 60-min open field session and graph showing total distance traveled (cms) measured in 10-min time bins across a 60-min session in an open field box, Iqsec2+/Y (n=16) and Iqsec2−/Y (n=14), Two-way ANOVA, ***p < 0.001; error bars indicate ± SEM. C. Time spent in junction, percent time spent in open arms, percent time spent in closed arms and total number of open and closed arm entries by Iqsec2+/Y (n=16) and Iqsec2−/Y (n=14) mice during 5 min exploration in elevated plus maze. Mann Whitney U test *p< 0.05, **p < 0.01; error bars indicate ± SEM. D. Total number of USV calls emitted by Iqsec2+/Y (n=10) and Iqsec2−/Y (n=12) mice when introduced to a novel estrous female during a social interaction session; Mann Whitney U test, ****p < 0.0001; error bars indicate ± SEM.