Effects of sepsis on the phenotype of non-cholinergic and cholinergic splenocytes determined by flow cytometry at 16 h post-surgery. These experiments were done using male ChATBAC-eGFP mice. A: Flow cytometry gating strategy and representative raw flow cytometry data. Phenotyping antibody vs. side scatter graphs were constructed, and the specific cell population gates were identified. Histogram plots of GFP fluorescence were then constructed using these gates. B: CLP did not affect the gross phenotype distribution of splenocytes. C: CLP increased the percentage of monocytes/macrophages that were cholinergic (i.e., GFP+). D: CLP did not affect the intensity of GFP fluorescence in cholinergic splenocytes, significantly, but there was an upward trend for the monocyte/macrophage population (P = 0.1324). For each cell type and parameter, Sham and CLP values were compared using an unpaired t-test. Values are means ± SEM, with n = 5 for Sham and n = 6 for CLP. *P < 0.05 vs Sham.