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. 2020 Jun 24;12:54. doi: 10.1186/s13073-020-00750-5

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Quantification of nucleosome organization strength. The summed WPS₁₂₀ for all points in the green shaded area (∑P_10..60) is subtracted from the summed WPS₁₂₀ for all points in the red shaded area (∑P_135..185). Different TSS-offsets must be used if a different WPS window length is chosen. A high value of this metric represents a more highly organized nucleosome positioning at the studied transcription start sites