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. 2020 Jun 24;20:274. doi: 10.1186/s12935-020-01306-8

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 3 — Cytoplasmic LINC01094 was an endogenous sponge of miR-577 in ccRCC. a lncLocator prediction of LINC01094 localization. b The specific distribution of LINC01094 in Caski and 786-O cells was determined via subcellular fractionation and qRT-PCR. c The expression of miR-577 in these ccRCC cell lines was tested by qRT-PCR. d qRT-PCR result of miR-577 level in Caski and 786-O cells with or without LINC01094 inhibition. e The expression of miR-577 or LINC01094 in Caski and 786-O cells with the transfection of miR-NC or miR-577 mimics was analyzed by qRT-PCR. f, g The binding sequences and results of luciferase reporter assay in HEK-293T cells. h RIP assay uncovered the co-enrichment of LINC01094 and miR-577 in Ago2-assembled RISC in two ccRCC cells. *P < 0.05, **P < 0.01, ***P < 0.001