Figure 4.

Muscadine grape extract (MGE) inhibits c-Raf and RAF1. c-Raf protein was measured by western blot analysis in 4T1 (A), MDA-MB-231 (B), and BT-549 (C) cells treated with 10 µg phenolics/mL or 20 µg phenolics/mL of MGE for either 12 or 36 hours, as indicated. Representative bands are shown below the graphs for each cell type. Relative fold-change in band intensity compared with the control is shown below the western blot bands. 4T1 (D), MDA-MB-231 (E), and BT-549 (F) cells were treated for 1, 6, 12, or 24 hours with 20 µg phenolics/mL of MGE, and RAF1 was measured by qRT-PCR. n = 3 to 5; *P < .05, **P < .01, and ***P < .001.