Ddr2 knockout exhibits decreased osteogenic
and odontogenic differentiation of periodontal ligament
(PDL) and dental pulp cells, respectively.
(A, B) Representative images of
EdU staining for labeling proliferating cells (green) and
DAPI staining for cell nuclei (blue). (C)
Quantification of the percentage of EdU-positive cells.
(D, E) Representative
images of p-RUNX2 immunostaining (red) in the nuclei of
PDL cells and at the apex of the developing root.
(F) Quantification of the fluorescent
intensity of p-RUNX2 immunostaining, n =
3 mice. (G–J) Quantification of
differentiation markers for PDL cells. (K–N)
Quantification of differentiation markers for dental pulp
stromal cells after 21 d. *P < 0.05.
ns, not significant. n = 3 cell
cultures/group. Scale bar: 50 µm in A, B; 100 µm in D,
E.