Figure 2.
Dilution series comparing various RT-PCR assays for the detection of SARS-CoV-2 at different laboratories, Germany, March 2020 (n =11 test systems with 34 reaction–lab combinations)
Beta-CoV: Betacoronavirus; CDC: Centers for Disease Control and Prevention; E: envelope gene; N: nucleocapsid gene; ORF: open reading frame; RdRp: RNA-dependent RNA-polymerase gene; S: spike gene; SARS-CoV-2: severe acute respiratory syndrome coronavirus 2.
a Copy numbers are estimated based on ddPCR results shown in Figure 1.
A 1:10 dilution series was prepared from pooled eluates of one SARS-CoV-2 stool sample and sent to participating laboratories to be tested in quadruplicate. The number of positive replicates is indicated and colour-coded as a heatmap with four positive results shown in green and four negative results shown in red.
