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. 2020 Jun 24;40(6):BSR20201175. doi: 10.1042/BSR20201175

Figure 1. CRC cells lead to activation of osteoclasts and osteolysis in vivo.

Figure 1

(A) HE staining and Safranin O-Fast Green staining showing the trabecular area and Trap analysis showing the number of Trap (+) cells in bone metastasis of CRC over time after intratibially injection of MC-38 cells (black scale bar = 100 μm and white scale bar = 250 μm). (B) Quantification of trabecular area from Safranin O-Fast green staining in (A). (C) Quantification of osteoclast number in (A). (D) Trap staining revealing the effect of MC-38 CM on osteoclastogenesis of BMMs following stimulation with RANKL (50 ng/ml) (scale bar = 20 μm). (E) Quantification of nuclei number per osteoclast in (D). (F) Relative Trap activity assay showing the Trap activity in BMMs treated by MC-38 CM plus RANKL. Abbreviations: wks, weeks; Ctrl, control; CM, conditional medium; No., number. *P<0.05, **P<0.01, ***P<0.001.