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LC-MS (A) and GC-MS (C) profiles showing the formation of an anhydro form of Neu5Ac (m/z = 290.08814, B) upon incubation of 1 mM Neu5Ac with YjhC (1.49 µM), with or without 30 µM NAD+. This anhydro form coelutes with 2,7-AN and not with the 2,3-EN. One representative experiment is shown.
