Figure 6. Time course of the formation of NADH and reaction intermediates from Neu5Ac, 2,3-EN and 2,7-AN.

YjhC (0.45 µM) was incubated for the indicated times in the presence of 1 mM Neu5Ac (A–C), 2,3-EN (D–F) or 2,7-AN (G–I) and 30 µM NAD⁺. Same experiment as in Figure 3 and Supplementary Figure S4. The intermediates are designated by their rounded m/z values and their retention time.