Figure 3.
Total and phosphorylated RpS6 protein levels following motor learning. (A) Example Western blotting bands from striatum for phosphorylated and total RpS6 and actin following motor learning (ML) or the exercise control (Exc) condition. Western blotting immunoreactivity to total RpS6 was greater in striatum after motor learning compared to exercise control (B) without a significant increase in the ratio of phosphorylated RpS6 at site Ser240/244 (C). (D) Example Western blotting bands from cerebellum for phosphorylated and total RpS6 and actin following motor learning (ML) or the exercise control (Exc) condition. No differences in Western blotting immunoreactivity to total RpS6 (E) or phosphorylated RpS6 (F) were observed in the cerebellum following motor learning compared to the exercise control. N = 10 mice per condition. * p < 0.05.