FIGURE 4.

MRTF-A modulates histone modifications surrounding the iNOS promoter. (A–D) RAW264 cells were transfected with siRNA targeting MRTF-A or SCR followed by exposure to hypoxia-reoxygenation. ChIP assays were performed with anti-acetyl H3 (A), anti-acetyl H3K9 (B), anti-acetyl H3K27 (C), and anti-acetyl H4K16 (D). (E–H) RAW264 cells were treated with CCG-1423 and/or hypoxia-reoxygenation. ChIP assays were performed with anti-acetyl H3 (E), anti-acetyl H3K9 (F), anti-acetyl H3K27 (G), and anti-acetyl H4K16 (H). (I–L) Primary BMDMs were isolated from WT and MRTF-A KO mice and exposed to hypoxia-reoxygenation. ChIP assays were performed with anti-acetyl H3 (I), anti-acetyl H3K9 (J), anti-acetyl H3K27 (K), and anti-acetyl H4K16 (L). N = 3 for all the experiments. Data represent averages of three independent experiments and error bars represent SEM.