Figure 2.

Variation of pH (blue), G′ (black), G″ (red), and complex viscosity (green) with time for 1 from the enzymatic reaction in the absence (a) and presence (c) of methyl formate. (b) and (d) represent the phase change of 1 with time associated with the enzymatic reactions performed in the absence (a) and presence (c) of methyl formate, respectively. The white structures in the gels are air bubbles, not precipitation. For (a)–(d), the initial reaction conditions are as follows: [urease] = 0.2 mg/mL, [urea] = 0.02 M. Solvent is 20/80 DMSO/water (v/v), and [1] = 2 mg/mL. For (c) and (d), the volume of methyl formate used is 100 μL.